Direct activation of these pathways was carried out by transfection of a constitutively activated form of RIG-I (RIG-I-Nter) or by addition of supernatants containing IFN1, respectively. (A and C) Schematic representations of the RIG-I pathway leading to the induction of type-I IFN and the IFN I pathway leading to interferon-stimulated gene (ISG) expression. SAV2 nsP2 protein blocks RIG-I-induced activation of IFN1 and ISRE promoter. RIG-I SAV2 alphavirus interferon nsP2 salmonid. In the present study, we focus on the nonstructural protein nsP2 and characterize a specific domain containing nuclear localization sequences that are critical for the inhibition of the host innate immune response mediated by the RIG-I pathway. While the clinical and pathological signs of SAV infection are fairly well known, the molecular mechanisms involved are poorly described. Recurrent outbreaks of SAV result in significant economic losses with serious environmental consequences for wild stocks. Aquaculture is currently the world's fastest-growing food production sector, with an annual growth rate of 6 to 8%. IMPORTANCE The global consumption of fish continues to rise, and the future demand cannot be met by capture fisheries alone due to limited stocks of wild fish. These results therefore demonstrate a major role for nsP2 in the control by SAV of the host cell's innate immune response. Finally, nsP2 does not interact with IRF3, indicating that its action is possible through a targeted interaction within discrete areas of chromatin, as suggested by its punctate distribution observed in the nucleus. Mutation of the NLS of nsP2 is deleterious to the virus. The C-terminal domain of nsP2 by itself is sufficient and necessary to block IFN induction. The inhibitory effect of nsP2 on the RIG-I pathway depends on the localization of nsP2 in the host cell nucleus, which is linked to two nuclear localization sequences (NLS) located in its C-terminal part. This inhibition, independent of the protease activity carried by nsP2, occurs downstream of IRF3, which is the transcription factor allowing the activation of the IFN promoter and its expression. By studying the role of SAV proteins on the RIG-I signaling cascade, the first line of defense of the immune system during infection, we demonstrated that nonstructural protein 2 (nsP2) effectively blocks the induction of type I interferon (IFN).

The mechanisms by which SAV escapes the host immune system remain unknown. Unlike other alphaviruses, such as the chikungunya virus, SAV is transmitted without an arthropod vector, and it does not cause cell shutoff during infection. Salmonid alphavirus (SAV) is an atypical alphavirus that has a considerable impact on salmon and trout farms.

Wild 9 rig